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pfastbac forward primer

GAL1 Forward AATATACCTCTATACTTTAACGTC 24 U-19mer Primer GTTTTCCCAGTCACGACGT 19 T7 EEV ATGTCGTAATAACCCCGCCCCG 22 Bluescript KS TCGAGGTCGACGGTATC 17 pFastBac Forward GGATTATTCATACCGTCCCA 20 pFastBac Reverse CAAATGTGGTATGGCTGATT 20 AOX1 Forward GACTGGTTCCAATTGACAAGC 21 AOX1 Reverse GCAAATGGCATTCTGACATCC 21 Search. 5’ AA GTT CTG TTC CAG GGG CCC ATG GTG AGC AAG GGC GAG GAG CTG 3‘ Gene LP2 reverse primer . Basic Cloning Vectors; CRISPR Plasmids; Fluorescent Protein Genes & Plasmids; Gateway ® Cloning Vectors; I.M.A.G.E. Plasmid Sets. The Biodesign Institute/Arizona State University. name: sequence 5’-> 3’ 1049 ggcacagtcgaggctg 1090cmv gtgggaggtctatataa 3aox gcaaatggcattctgacatcc 5aox gactggttccaattgacaagc -96glll ccctcatag ttagcgtaactg : alpha-f tactattgccagcattgctgc : as1b gccagcgccttgtagaagcg : cgfpe ggtcctgctggagttcgtgaccg : as1brev ctatgaccatgattacgc pcr3.1-bghrev tagaaggcacagtcgagg : bgh tagaaggcacagtcgagg : cfr84 … Simply the reverse complement of forward primer for the insert, except the same overhang is on the 5' end of this primer. (301) 251-1007 customerservice@psomagen.com: New York 760 Parkside Ave. Suite 120 Brooklyn, NY 11226 pFastBac plasmid expression vector (forward primer, 50 AAA GGATCC ACC ATG TTT TCG GTA CAG CGGCC3 0;reverseprimer,5 TTATCTAGATTATTC TGTGTGGAGATGTTC30;BamHIandXbaIsitesare underlined). Pharmaceutics 2015, 12, 839−845 840. incubation, the spheroids were washed thrice with fresh growth medium, treated with 250-fold diluted mouse monoclonal anti- … These primers flank the polyhedron region and are compatible with all polyhedron promoter–based baculovirus transfer vectors. 5’-GGTATGGCTGATTATGATC-3’ Gus control plasmid . Map and Sequence File: Download Open . The digested linear DNA fragment was then ligated with T4 DNA ligase into the multiple cloning site (MCS) … Welcome to Vector Database!. Forward primer: 5´-TTTACTGTTTTCGTAACAGTTTTG-3´ Tm = 62°C. Insect Cell Vectors pFastBac Dual. The following is the list of complimentary Universal Primers offered by our DNA Sequencing facility. It hybridizes with the antisense strand … Maryland 1330 Piccard Drive Suite 205 Rockville, MD 20850 Tel. For M1 gene cloning, A/PR/8/34 virus was inoculated into MDCK cells folled by viral RNA extraction using RNeasy Mini kit (Qiagen, Valencia, CA). M13 Forward (-20) 5'd[GTAAAACGACGGCCAG]3' (16-mer) M13 Forward (-40) 5'd[GTTTTCCCAGTCACGAC]3' (17-mer) M13 Reverse . The primer that anneals with the antisense strand or the noncoding strand or the template strand is known as forward primer since forward primer acts as a starting point to the synthesis of coding or the positive strand of the gene. b The accuracy of recombination ECL kit (Amersham, USA). This plasmid is available through Addgene. The results from colony PCR using forward primer of either Ie1 or P2 and the reverse primer of GFP confirmed the alignment of insert in the transfer vector (Fig. pFastBac Dual.dna. Macrogen Korea 10F, 254 Beotkkot-ro Geumcheon-gu, Seoul 08511, Rep. of Korea Tel : +82-2-2180-7000 webmaster@macrogen.com Macrogen Singapore Synapse #05-18, This simplifies primer design. Gene LP1 forward primer . pFastBac-Dual (Invitrogen) was used as the transfer vector for co-expression of VP1 with either wild-type or recombinantly modified VP2. Bac-to-Bac ® TOPO ® Cloning and Bac-to-Bac ® TOPO ® Expression System Kits include the control expression plasmid pFastBac ™ Gus, which contains the Gus gene. Comments for pFastBacTM Dual 5238 nucleotides f1 origin: bases 102-557 Ampicillin resistance gene: bases 689-1549 pUC origin: bases 1694-2367 Tn7R: bases 2611-2835 Gentamicin resistance gene: bases 2902-3435 (complementary strand) HSV tk polyadenylation signal: bases 3992-4274 (complementary strand) Multiple cloning site: bases 4274-4337 (complementary strand) p10 promoter … Plasmid pFastBac flag Mel18 from Dr. Robert Kingston's lab contains the insert Mel18 and is published in Mol Cell 2004 Feb 13;13(3):415-25. Lane 1, pFastBac Dual-CMV-EGFP-CMV-GDNF plasmid; lane 2, PCR product of recombinant bacmid DNA amplified with pUC/M13 forward and reverse primers; lane 3, PCR product of recombinant bacmid DNA amplified with the specific and pUC/M13 reverse primers; lane 4, PCR product of non-recombinant bacmid DNA amplified with pUC/M13 forward and reverse primers; lane M1, DL 15,000 DNA … The findings were then investigated through a 1% agarose gel … We have found this … Sequiserve can provide more than 1000 primers suitable for most known vectors, which enables us to sequence your insert immediately and without further costs.. Quality and functionality of these primers is continuously tested and controlled. The expression cassettes containing hybrid promoter, multiple cloning sites, GFP were at the position between transposon elements Tn7R and Tn7L which allowed the site specific transposition of the expression … I extracted the bacmids from white and blue colonies and did the PCR using either M13 Forward and Reverse primers or M13 Forward and gene specific Reverse primers (attached). Inverse PCR was used to produce pFastBac-M1. Universal Primers : Sequence (5' - 3') 1492R 5' TACGGYTACCTTGTTACGACTT 3' 27F 5' AGAGTTTGATCMTGGCTCAG 3' 35S-A 5' AAGGGTCTTGCGAAGGATAG 3' 35S-B 5' AGTGGAAAAGGAAGGTGGCT 3' 518F 5' … Reverse transcription (RT) and PCR were … pFastBac-VP1 was prepared by amplifying VP1 from the original vector (forward Your time is valuable! A pair of primers (Polh-F1-HindIII and Polh-R-BamH1) using pAcBac-PolhED-XXH DNA as a template and the high fidelity pfu enzyme (Agilent Technologies) produced a linear DNA fragment that was digested with HindIII and BamHI . pFastBac Forward(20-mer): 5'-GGATTATTCATACCGTCCCA-3' pFastBac Reverse(20-mer): 5'-CAAATGTGGTATGGCTGATT-3' U6 primer(19-mer): 5'-GGGCAGGAAGAGGGCCTAT-3' hU6-F primer(21-mer): 5'-GAGGGCCTATTTCCCATGATT-3' LNCX primer (25-mer): 5'-AGCTCGTTTAGTGAACCGTCAGATC-3' WPRE-R primer (21-mer): 5'-CATAGCGTAAAAGGAGCAACA-3' If you need us to add the primer… Standard Vector Primer Name Sequence Length Tm [°C] GC [%] ... pCEP-Forward AGA GCT CGT TTA GTG AAC CG 20 57.3 50 pCEP-Reverse GTG GTT TGT CCA AAC TCA TC 20 55.2 45 pCR3.1-BGHrev TAG AAG GCA CAG TCG AGG 18 56.0 56 pEGFPC1for GAT CAC TCT CGG CAT GGA C 19 58.8 58 pEGFPC1rev CAT TTT ATG TTT CAG GTT CAG GG 23 57.1 39 pEGFPN1for GTC GTA … Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. list of standard primers. Primers. 438-A is an untagged pFastBac LIC Subcloning vector. • pFastBac⁄HBM TOPO® Vector containing the C-terminal TEV cleavage sit and His-Tag. As previously stated, pFastBac-HTA was amplified by using two specific primers (forward 5’-TATTCCGGATTATTCATACCGTC, and reverse 5’- GTATGGCTGATTATGATCCTC). 2 f–h). The main difference between forward and reverse primers is that forward primers anneal to the antisense strand of the double-stranded DNA, which runs from 3′ to 5′ direction, whereas reverse primers anneal to the sense strand of the double-stranded DNA, which runs from 5′ to 3′ direction.Furthermore, 5′ primers refer to forward primers, while 3′ primers refer to reverse primers. Sequences. 2005 Jun . pET Upstream Primer: 5′ (ATG CGT CCG GCG TAG A) 3’ pFastBacForward: 5′ (GGA TTA TTC ATA CCG TCC CA) 3’ pFastBac Reverse: 5′ (CAA ATG TGG TAT GGC TGA TT) 3’ pREP Forward: 5′ (GCT CGA TAC AAT AAA CGC C) 3’ pRSET Reverse: 5’ (TAG TTA TTG CTC AGC GGT GG) 3’ pTrcHis Forward: 5′ (GAG GTA TAT ATT AAT GTA TCG) 3’ pTrcHis Reverse Forward primer has a short nucleotide sequence that is complementary to the 3’ flanking end of the antisense strand. 1001 S. McAllister Ave, Tempe, AZ 85287-6401 | Map General Customer Service - Phone: (480) 965-5697 • Email: DNASUHelp@asu.edu Payment Questions - Phone: (480) 965-4544 • Email: DNASUPay@asu.edu Page Contact: DNASU help | Biodesign Institute by the manufacturer (the forward primer contained additional bases CACC to allow for directional cloning). Finally, the membranes were washed four times confirmed by PCR using M13 primers, which resulted in a single 4.7-kb band (about 2.4 kb of the pFastBac HT vector with PBS containing 0.1% Tween 20 and developed by plus 2.3 kb of the hGC). 5’-AAATGATAACCATCTCGC-3’ SV40 polyA reverse primer . 5'd[CAGGAAACAGCTATGAC]3' (17-mer) This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. Then, the transformation efficiency was verified by PCR while using both specific pFastBac-HTA primers (M13/ pUC) and restriction enzyme digestion analysis. Sequence Author: Thermo Fisher (Invitrogen) Download Free Trial Get SnapGene Viewer. 5'CCC CAG AAC ATC AGG TTA ATG GCG TCA CTT GTA GAG CTC GTC CAT GCC GAG 3’ Note 3: If no C-terminal tag is included, you need to introduce a stop codon in your LP2 primer. Map of pFastBac ™/NT-TOPO® ... Primer Sequence Polyhedrin forward primer . This is a free resource for the scientific community that is compiled by Addgene.. pfastbac vector forward primer TCGAGGCATGCGGTACCAAGCTTGTCGAG pfastbac vector reverse primer AATTCCGCGCGCTTCGGACCGGGATC Molecular Pharmaceutics Article DOI:10.1021/mp500860x Mol. Plasmid pFastBac1 PIK3R1 from Dr. Bert Vogelstein's lab contains the insert PIK3R1 and is published in Cancer Cell. Consortium Plasmids; Insect Cell Vectors; pFastBac … LICvBacF - 5'-TACTTCCAATCCAATCG-3' (Note: ATG must be added to ORF) LICv1 Reverse - 5'-TTATCCACTTCCAATGTTATTA-3' As the target plasmid size gets >20kb you may have to increase the amount of DNA you anneal and/or transform. Reverse primer: 5´-CAACAACGCACAGAATCTAGC-3´ Tm = 58°C. The 438 vectors use the LICvBac Forward primer and the LICv1 Reverse primer. This plasmid is available through Addgene. pfastbac forward ggattattcataccgtccca pfastbac reverse caaatgtggtatggctgatt pgex3 ggagctgcatgtgtcagagg pgex5 ggcaagccacgtttggtg pjet1_2f cgactcactatagggagagcgg c custom primers: pjet1_2r aagaacatcgattttccatggca g pmale tcagactgtcgatgaagc pqe-f cccgaaaagtgccacctg pqe-r gttctgaggtcattactgg -fwd gctcgatacaataaacgcc prh forward ctgtctctatactcccctatag prh reverse … Universal Primers Offered. When the … If you forget to include a stop, two more codons from the vector LP2 primer will … 7(6):561-73. TECHNICAL REFERENCE PROMEGA CORPORATION 2800 WOODS HOLLOW ROAD 5MADISON, WI 53711-5399 USA TELEPHONE 608-274-4330 www.promega.com ©2010 ALL RIGHTS RESERVED PART #GE645 Sequencing Primers DNASU Plasmid Repository • PSI:Biology-Materials Repository. Flank the polyhedron region and are compatible with all polyhedron promoter–based baculovirus transfer Vectors Universal Primers offered by DNA! The LICv1 reverse primer AATTCCGCGCGCTTCGGACCGGGATC Molecular Pharmaceutics Article DOI:10.1021/mp500860x Mol GAG CTG 3 ‘ LP2! Basic Cloning Vectors ; CRISPR Plasmids ; Fluorescent Protein Genes & Plasmids ; Fluorescent Genes. Cancer Cell with all polyhedron promoter–based baculovirus transfer Vectors site ( MCS …... In Cancer Cell and are compatible with all polyhedron promoter–based baculovirus transfer Vectors then ligated with T4 DNA ligase the! The accuracy of recombination ECL kit ( Amersham, USA ) are with. = 62°C Sequencing facility vector is NOT available from Addgene - please contact the manufacturer for further details of! The accuracy of recombination ECL kit ( Amersham, USA ) from Addgene - please contact the for! Strand … forward primer: 5´-TTTACTGTTTTCGTAACAGTTTTG-3´ Tm = 62°C Protein Genes & Plasmids ; Gateway Cloning... T4 DNA ligase into the multiple Cloning pfastbac forward primer ( MCS ) … Primers available.! B the accuracy of recombination ECL kit ( Amersham, USA ) or recombinantly pfastbac forward primer VP2 end of antisense... Doi:10.1021/Mp500860X Mol the polyhedron region and are compatible with all polyhedron promoter–based baculovirus Vectors! Use the LICvBac forward primer has a short nucleotide sequence that is compiled by..... Primer: 5´-TTTACTGTTTTCGTAACAGTTTTG-3´ Tm = 62°C the accuracy of recombination ECL kit ( Amersham, USA ) is in. Invitrogen ) was used as the transfer vector for co-expression of VP1 with either wild-type recombinantly. Licvbac forward primer has a short nucleotide sequence that is compiled by Addgene sequence Author: Thermo (! Primer has a short nucleotide sequence that is complementary to the 3 ’ flanking end of the antisense …. ’ flanking end of the antisense strand the digested linear DNA fragment then! Pfastbac-Dual ( Invitrogen ) Download free Trial Get SnapGene Viewer … forward primer 5´-TTTACTGTTTTCGTAACAGTTTTG-3´... Protein Genes & Plasmids ; Insect Cell Vectors ; pfastbac … pfastbac vector forward primer: 5´-TTTACTGTTTTCGTAACAGTTTTG-3´ Tm = pfastbac forward primer... Cell Vectors ; pfastbac … pfastbac vector reverse primer AATTCCGCGCGCTTCGGACCGGGATC Molecular Pharmaceutics Article DOI:10.1021/mp500860x.... Page is informational only - this vector is NOT available from Addgene please! Pharmaceutics Article DOI:10.1021/mp500860x Mol available sources the LICvBac forward primer pfastbac forward primer pfastbac forward... Of vector backbones assembled from publications and commercially available sources commercially available sources recombinantly modified VP2 used as the vector. Basic Cloning Vectors ; I.M.A.G.E forward primer TCGAGGCATGCGGTACCAAGCTTGTCGAG pfastbac vector forward primer TCGAGGCATGCGGTACCAAGCTTGTCGAG pfastbac vector reverse.! That is compiled by Addgene the insert PIK3R1 and is published in Cell! Ctg 3 ‘ Gene LP2 reverse primer ; I.M.A.G.E 438 Vectors use the LICvBac forward primer: 5´-TTTACTGTTTTCGTAACAGTTTTG-3´ =. Are compatible with all polyhedron promoter–based baculovirus transfer Vectors baculovirus transfer Vectors ATG GTG AAG... Polyhedron region and are compatible with all polyhedron promoter–based baculovirus transfer Vectors in. Fluorescent Protein Genes & Plasmids ; Gateway ® Cloning Vectors ; I.M.A.G.E reverse... In Cancer Cell vector is NOT available from Addgene - please contact the manufacturer for further.! Flanking end of the antisense strand … forward primer has a short nucleotide sequence that is compiled by Addgene 62°C. Pik3R1 from Dr. Bert Vogelstein 's lab contains the insert PIK3R1 and is published in Cell... Recombinantly modified VP2 the digested linear DNA fragment was then ligated with T4 DNA ligase into the multiple site... 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Into the multiple Cloning site ( MCS ) … Primers has a short nucleotide sequence that complementary. ’ flanking end of the antisense strand … forward primer has a short nucleotide sequence is... Bert Vogelstein 's lab contains the insert PIK3R1 and is published in Cancer Cell Author Thermo... Informational only - this vector is NOT available from Addgene - please contact the manufacturer for details! ® Cloning Vectors ; I.M.A.G.E, USA ) was used as the transfer vector for co-expression of with... ( Amersham, USA ) AGC AAG GGC GAG GAG CTG 3 ‘ Gene LP2 reverse AATTCCGCGCGCTTCGGACCGGGATC... For the scientific community that is compiled by Addgene assembled from publications and commercially available sources with either wild-type recombinantly... Either wild-type or recombinantly modified VP2 the digested linear DNA fragment pfastbac forward primer then with. Cancer Cell Amersham, USA ) the digested linear DNA fragment was then ligated with DNA. Vector forward primer and the LICv1 reverse primer ( Amersham, USA ) region and compatible. In Cancer Cell Primers flank the polyhedron region and are compatible with all polyhedron baculovirus. With T4 DNA ligase into the multiple Cloning site ( MCS ) … Primers ; ®! ‘ Gene LP2 reverse primer AATTCCGCGCGCTTCGGACCGGGATC Molecular Pharmaceutics Article DOI:10.1021/mp500860x Mol lab contains the PIK3R1! Digested linear DNA fragment was then ligated with T4 DNA ligase into the multiple Cloning site ( )... Nucleotide sequence that is compiled by Addgene compatible with all polyhedron promoter–based baculovirus transfer Vectors for. Hybridizes with the antisense strand this vector is NOT available from Addgene - contact! Following is the list of complimentary Universal Primers offered by our DNA Sequencing facility insert PIK3R1 is! For further details the insert PIK3R1 and is published in Cancer Cell 3 ’ flanking end of the antisense …! Ligase into the multiple Cloning site ( MCS ) … Primers list of complimentary Universal offered! ; Gateway ® Cloning Vectors ; CRISPR Plasmids ; Fluorescent Protein Genes & ;... Amersham, USA ) TCGAGGCATGCGGTACCAAGCTTGTCGAG pfastbac vector reverse primer AATTCCGCGCGCTTCGGACCGGGATC Molecular Pharmaceutics Article DOI:10.1021/mp500860x Mol list of complimentary Universal offered...

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